The True Identity of Zangetsu: One of the biggest twists is that the "Old Man Zangetsu" Ichigo conversed with was never his Soul Reaper power, but the manifestation of his Quincy powers (taking the form of a young Yhwach). His true Soul Reaper power was the "Inner Hollow" (White). This recontextualizes the entire series: the Old Man was suppressing Ichigo's power to protect him from becoming a Soul Reaper and facing Yhwach.
The Soul King's Nature: It is revealed that the Soul King is not a ruler but a linchpin—a sealed, limbless entity kept in a state of living death by the Soul Reapers to maintain the separation of worlds. This justifies Aizen's disgust and rebellion.
Unohana's Secret: The gentle healer Captain Retsu Unohana is revealed to be the first "Kenpachi," the most bloodthirsty criminal in Soul Society history, who trains Zaraki to unlock his true potential by killing him and reviving him repeatedly.