Evangelion: 3.0+1.0 Thrice Upon a Time finally unravels Gendo Ikari's ultimate goal. His plan was to use Eva-13, the Spears of Longinus and Cassius, and the Human Instrumentality Project to trigger an "Additional Impact" within a metaphysical "Anti-Universe." This would allow him to rewrite reality itself and reunite with his deceased wife, Yui. He reveals he used the Key of Nebuchadnezzar to transcend humanity, making him immune to harm.
A significant revelation is that Asuka is part of the "Shikinami series" of clones, similar to Rei's Ayanami series, explaining her desperation to be an "original." To fight NERV, she removes her eyepatch, which was sealing a parasitic Angel within her, briefly granting her immense power before she is absorbed by Eva-13.
The climax is a psycho-analytical confrontation between Shinji and Gendo inside the Anti-Universe. Instead of a purely physical battle, they talk, and Shinji comes to understand his father's profound loneliness and grief, realizing they are very much alike. This empathy gives Shinji the strength to take control. With the help of a resurrected Misato, who sacrifices herself and the AAA Wunder to create a new spear, the "Lance of Gaius," Shinji decides against Gendo's plan of escapism. He instead performs his own version of Instrumentality to give closure to his friends: he acknowledges his past feelings for Asuka, freeing her; he allows Kaworu to find peace outside of his connection to him; and he gives the original Rei Ayanami the chance for a normal life.
Ultimately, Shinji makes the final choice: to create a "Neon Genesis," a new world completely free of Evangelions. He intends to sacrifice himself to complete this rewriting, but his parents, Gendo and Yui, intervene and take his place, finally together in the end. Shinji is left on a beach in the new world until Mari arrives to pick him up. The final scene jumps to a train station, where adult versions of all the pilots are seen, having lived full lives. Shinji and Mari leave together, hand-in-hand, as the animation gives way to live-action, signifying their complete entry into the real world.