"Pather Panchali" culminates in a series of tragedies that shatter the family's life in Nischindipur. The first major loss is the death of the elderly cousin, Indir Thakrun. After a bitter argument, Sarbajaya forces her out of the house. Indir dies alone in the wilderness, and her body is discovered by the children, giving them their first direct encounter with death.
The film's central tragedy is the death of Durga. During a powerful monsoon, Durga plays joyfully in the downpour but catches a severe fever. With limited resources and her father Harihar away seeking work, her condition worsens. She dies during the storm-wracked night. The hidden meaning that becomes clear only after this is the finality of her unfulfilled dreams, symbolized by her last wish to see the train again.
Harihar returns home days later, cheerful and oblivious, bringing gifts for his family, including a sari for Durga. When he learns of his daughter's death, he collapses in grief. This event is the final blow to the family's tenuous hold on their ancestral life. While packing to leave the village for good, Apu discovers a bead necklace that Durga had stolen but always denied taking. In a moment of profound, quiet understanding, Apu throws the necklace into a pond, concealing his sister's secret and letting go of the last remnant of their shared childhood. The film ends with the surviving family members—Harihar, Sarbajaya, and Apu—leaving Nischindipur on an ox-cart, their faces set towards an uncertain future.